Defective Control of Mitotic and Post-mitotic Checkpoints in Poly(ADP-ribose) Polymerase-1-/- Fibroblasts After Mitotic Spindle Disruption
نویسندگان
چکیده
منابع مشابه
Mitotic checkpoints, MUGs and kinetochores
Positioning of the cell division plane is critically important for tissue morphogenesis and architecture. 1 it is therefore not surprising that mitotic spindle orientation must be tightly regulated in living tissues, a phenomenon that is also observed in cells cultured in vitro. Because of the amenability of cultured cells to molecular and physical manipulation, many investigators have used suc...
متن کاملThe mitotic spindle checkpoint
One of the most awe-inspiring demonstrations in biology is a timelapse video of mitosis viewed with high magnification phase contrast or Nomarski DIC microscopy. From initial chaotic movement comes the orderly alignment of chromosomes at the metaphase plate followed by a flawlessly synchronous splitting of the chromosomes and their gliding separation to the two mitotic poles. Enforcing the timi...
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Dynein motor isoforms have been implicated as potential kinetochore-associated motors that power chromosome-to-pole movements during mitosis. The recent identification and sequence determination of genes encoding dynein isoforms has now permitted the in vivo analysis of dynein function in mitosis. In this report we describe the identification and mutational analysis of the gene, DHC1, encoding ...
متن کاملStructure of the Mitotic Spindle in L Strain Fibroblasts
The mitotic spindle of L strain fibroblasts, fixed with glutaraldehyde followed by osmium tetroxide, contains many 150- to 180-A tubules. They appear first in the cytoplasm. They extend from the centrospheres to the kinetochores, and from one centrosphere to the other. Only occasionally can points of continuity between the spindle tubules and the tubules of the centrioles be observed. The chrom...
متن کاملMitotic spindle form and function.
The Saccharomyces cerevisiae mitotic spindle in budding yeast is exemplified by its simplicity and elegance. Microtubules are nucleated from a crystalline array of proteins organized in the nuclear envelope, known as the spindle pole body in yeast (analogous to the centrosome in larger eukaryotes). The spindle has two classes of nuclear microtubules: kinetochore microtubules and interpolar micr...
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ژورنال
عنوان ژورنال: Cell Cycle
سال: 2004
ISSN: 1538-4101,1551-4005
DOI: 10.4161/cc.3.3.670